The composition of the gut microbiome is altered by many factors including, but not limited to, diet, exercise, and antibiotic use. Now, the first major paper from the Global Microbiome Conservancy (GMbC), a consortium that is collecting microbiome samples from underrepresented human populations around the world in an effort to preserve bacterial species that are at risk of being lost as humanity becomes more exposed to industrialized diets and lifestyles globally, shows how industrialization has impacted the human gut ecosystem, resulting in altered microbiome composition and diversity.
The new study from an MIT-led team has revealed that the microbiome can remake themselves within the lifetime of their host by passing genes back and forth. The researchers also showed that this kind of gene transfer occurs more frequently in the microbiomes of people living in industrialized societies, possibly in response to their specific diets and lifestyles.
This work is published in Cell in the article, “Elevated rates of horizontal gene transfer in the industrialized human microbiome.”
“One unexpected consequence of humans living in cities may be that we’ve created conditions that are very conducive to the bacteria that inhabit our guts exchanging genes with each other,” said Eric Alm, PhD, director of MIT’s Center for Microbiome Informatics and Therapeutics and the senior author of the new study. But whether bacterial genomes may also adapt to the industrialization of their host populations remains largely unexplored.
Global Microbiome Conservancy co-founder Mathieu Groussin, PhD, collects dietary data from Hadza villagers in Tanzania. [Global Microbiome Conservancy; Photo by C. Corzett]
“Most of the species that we find in rural and isolated populations are species that you wouldn’t see in the industrialized world,” said Mathieu Groussin, PhD, a post-doc in the Alm lab and one of the lead authors of the paper. “The composition of the microbiome shifts completely, and along with this, the number of different species is diminishing. This lower diversity of the industrialized microbiome might be a reflection of poor intestinal health.”
The GMbC launched in 2016, with the mission of preserving human microbiome diversity before it is lost. So far, the project has gathered samples from 34 human populations worldwide. The GMbC consortium includes scientists from each country where samples are being collected. Indeed, other authors of the paper include researchers from institutions in Denmark, France, South Africa, Cameroon, Canada, Finland, New Zealand, Tanzania, Spain, Sweden, Ghana, and Nigeria.
Previous work has shown that the composition of the microbiome in people living in industrialized societies is very different from that of rural peoples living in relative isolation. Nonindustrialized populations usually have a larger bacterial biodiversity, including many species that are not seen in industrialized populations.
This study sought to investigate “the extent to which the rates and targets of horizontal gene transfer (HGT) vary across thousands of bacterial strains from 15 human populations spanning a range of industrialization.” In 2011, Alm’s lab discovered that the human gut is a hotspot for this type of gene exchange. However, with the technique the researchers were using at the time, they were only able to determine that these gene transfers had likely happened sometime within the past 5,000 years.
In their new study, the researchers were able to estimate much more precisely when these transfers occurred. To do this, they compared the genetic differences between different species of gut bacteria. When they compared pairs of bacterial species that came from the same person, they found a much higher rate of genetic similarity than that seen in the same pairs taken from two different people, confirming that horizontal gene transfer can happen within the lifetime of an individual person.
Global Microbiome Conservancy co-founder Mathilde Poyet, PhD, stores microbiome samples in a cryogenic tank containing liquid nitrogen during a field trip in Rwanda. [Global Microbiome Conservancy; Photo by C. Corzett]
“One of the really exciting things about this paper is we were finally able to answer the question of whether the rate of horizontal transfer has been high in the human microbiome over the last few millennia, or is it true that within each person’s lifetime, the bugs in their gut are constantly trading genes back and forth with each other,” Alm said.
Depending on the species, the researchers found that bacteria might obtain between 10 and 100 new genes every year. The researchers also found that the rate of gene exchange was significantly higher in people living in industrialized societies, and they also saw differences in the types of genes that were most commonly exchanged.
As one example, they found that among pastoralist populations who treat their livestock with antibiotics, genes for antibiotic resistance are among those exchanged at the highest rates. They also found that people from nonindustrialized societies, especially hunter-gatherers, had high rates of gene exchange for genes that are involved in fiber degradation. This makes sense because those populations usually consume much more dietary fiber than industrialized populations, the researchers said.
Among the microbes found in industrialized populations, the researchers found especially high rates of exchange for genes whose role is to facilitate gene transfer. These microbes also have higher exchange rates for genes involved in virulence. The researchers are now investigating how those genes may influence inflammatory diseases such as irritable bowel syndrome, which is seen much more often in industrialized societies than nonindustrialized societies.